abstract：:The effect of SM-10661, a selective antagonist of platelet-activating factor (PAF), on passive anaphylactic bronchoconstriction was examined in guinea pigs. A challenge of ovalbumin to passively sensitized guinea pigs induced bronchoconstriction, which peaked at 4 min. When SM-10661 was administered intravenously 2 mi...

journal_title：Lipids

authors： Uchida M,Imanishi N,Sugasawa T,Morooka S

更新日期：1991-12-01 00:00:00

abstract：:We have reported previously that platelet-activating factor (PAF) interacts with the neuronal cell line NG108-15 (neuroblastoma X glioma hybrid) and the pheochromocytoma cell line, PC12. PAF acts on these cells by raising levels of intracellular free calcium ions. In the present report, we extend these studies. PAF in...

journal_title：Lipids

authors： Kornecki E,Ehrlich YH

更新日期：1991-12-01 00:00:00

abstract：:A specific radioimmunoassay for platelet-activating factor (PAF) sensitive in the range 10-1000 pg (0.02-2 pmoles) has been developed. Detailed quantitative hapten inhibition studies showed specificity for the acetyl group at C-2 of PAF, a requirement for the ether linkage at C-1 and some tolerance for substituents on...

journal_title：Lipids

authors： Baldo BA,Smal MA,McCaskill AC

更新日期：1991-12-01 00:00:00

abstract：:Stimulation of platelets induces a rapid release of arachidonate from specific phospholipids and subsequent remodeling of arachidonate-containing phospholipids. This process is accompanied by transformation of released arachidonate by cyclooxygenase and lipoxygenase enzymes. We addressed the question of whether the cy...

journal_title：Lipids

authors： Chevy F,Wolf C,Colard O

更新日期：1991-12-01 00:00:00

abstract：:We have examined the possibility that human polymorphonuclear cells exposed to IgA immune complexes can mediate the production of platelet-activating factor (PAF) and oxygen radicals. We found that human IgA and IgG immune aggregates stimulated, to a similar extent, PAF and O2- production by human polymorphonuclear ce...

journal_title：Lipids

authors： Hernando P,Egido J,de Nicolas R,Gonzalez E

更新日期：1991-12-01 00:00:00

abstract：:Various derivatives and isosteres of neolignans of the 2,5-diaryl tetrahydrofuran type have been synthesized as antagonists of platelet-activating factor (PAF). A detailed analysis of their structure-activity relationship (SAR) has revealed a clear preference for an asymmetrical molecular configuration with a high deg...

journal_title：Lipids

authors： Shen TY

更新日期：1991-12-01 00:00:00

abstract：:The activity of the platelet-activating factor (PAF)-synthesizing enzyme, 1-O-alkyl-sn-glycero-3-phosphocholine (lysoPAF):acetyl-CoA acetyltransferase (EC 2.3.1.67) in alveolar macrophage lysate was found to be elevated after warming the cells to 37 degrees C. Such an increase in enzyme activity was detectable only wh...

journal_title：Lipids

authors： Sugiura T,Fukuda T,Cheng NN,Waku K

更新日期：1991-11-01 00:00:00

abstract：:A rat liver cytosolic cholesteryl ester hydrolase (CEH) was purified 12,600-fold by ammonium sulfate precipitation, cation exchange chromatography and gel permeation high-performance liquid chromatography, with an overall yield of 20%. Its properties are compared to those of pancreatic CEH, with which it has sometimes...

journal_title：Lipids

authors： Ghosh S,Grogan WM

更新日期：1991-10-01 00:00:00

abstract：:Meadowfoam oil is unusual because over 95% of the fatty acids are 20- and 22-carbon aliphatic acids with cis double bonds located principally at the 5- and/or 13-position. Since little information is available on the metabolism of the 5c-20:1 and 5c,13c-22:2 fatty acids, an exploratory study in mice was conducted to i...

journal_title：Lipids

authors： Emken EA,Adlof RO,Abraham S

更新日期：1991-09-01 00:00:00

abstract：:The resolution of a series of racemic 1-alkyl-3-trityl-rac-glycerols and 1-alkyl-2-benzyl-rac-glycerols into their enantiomers by high-performance liquid chromatography (HPLC) was investigated. Of the racemic glycerol derivatives studied, only three (1,2-0-isopropylidene-rac-glycerol, 1,2-0-isopropylidene-3-benzyl-rac...

journal_title：Lipids

authors： Foglia TA,Maeda K

更新日期：1991-09-01 00:00:00

abstract：:Yeast mutant strains auxotrophic for ergosterol and blocked in mevalonate diphosphate decarboxylase (erg19) and farnesyl diphosphate (FPP) synthetase (erg20) were isolated. The main feature of the mutants blocked in FPP synthetase is their ability to excrete prenyl alcohols, such as geraniol and farnesol. The isolatio...

journal_title：Lipids

authors： Chambon C,Ladeveze V,Servouse M,Blanchard L,Javelot C,Vladescu B,Karst F

更新日期：1991-08-01 00:00:00

abstract：:We investigated the effect of oral supplementation with evening primrose oil, containing 72% linoleic acid (18:2n-6) and 10% gamma-linolenic acid (18:3n-6), on the epidermal and neutrophil phospholipid fatty acid composition in 15 patients with atopic dermatitis (AD). Three different dose levels, 4, 8 and 12 capsules ...

journal_title：Lipids

authors： Schäfer L,Kragballe K

更新日期：1991-07-01 00:00:00

abstract：:Earlier studies showed that during the first 20 to 25 seconds of aggregation induced by thrombin (0.1 U/mL) or adenosine diphosphate (ADP) (2 microM) of rabbit or human platelets prelabeled with [3H]palmitic acid, labeled lipid became associated with the cytoskeleton (isolated after lysis with 1% Triton X-100, 5 mM EG...

journal_title：Lipids

authors： Packham MA,Guccione MA,Bryant NL,Livne A

更新日期：1991-07-01 00:00:00

abstract：:An improved headspace capillary gas chromatographic (GC) method was developed to measure the oxidative susceptibility of human red blood cell (RBC) membranes. This method analyzed volatile peroxidation products of both n-6 (hexanal and pentane) and n-3 (propanal) polyunsaturated fatty acids. Oxidative susceptibility t...

journal_title：Lipids

authors： Frankel EN,Tappel AL

更新日期：1991-06-01 00:00:00

abstract：:To examine whether dietary fat alters membrane lipid composition and peroxidation of polyunsaturated fatty acids in "non-proliferative" and "proliferative" cells in the large intestine, Sprague-Dawley rats were fed diets providing a polyunsaturated-to-saturated fatty acid ratio of 1.2 or 0.3 at a high or low level of ...

journal_title：Lipids

authors： Turini ME,Thomson AB,Clandinin MT

更新日期：1991-06-01 00:00:00

abstract：:The effects of aging on the hepatic metabolism of cholesterol were studied in 1-, 6- and 24-month-old male Sprague-Dawley rats. Microsomal 3-hydroxy-3-methylglutaryl coenzyme A (HMG-CoA) reductase activity, which regulates cholesterol biosynthesis, decreased from 835 +/- 144 (SEM) pmol/min/mg protein in the youngest g...

journal_title：Lipids

authors： Ståhlberg D,Angelin B,Einarsson K

更新日期：1991-05-01 00:00:00

abstract：:It has been shown that different lysophosphatidylcholines (LPC) possess relaxing properties in vascular strips. We report on the effect of intraatrial injection of LPC micelles into white New Zealand rabbits. Changes in renal and coronary flow were determined by radioactive microspheres. Coronary blood flow increased ...

journal_title：Lipids

authors： Wolf A,Saito T,Dudek R,Bing RJ

更新日期：1991-03-01 00:00:00

abstract：:The effects of n-3 fatty acids on plasma lipids, lipoproteins and apoproteins have usually been studied in humans after feeding of purified fish oil. This study describes the effect of a natural diet, containing salmon as the source of n-3 fatty acids, on these parameters as compared to a diet very low in n-3 fatty ac...

journal_title：Lipids

authors： Lindgren FT,Adamson GL,Shore VG,Nelson GJ,Schmidt PC

更新日期：1991-02-01 00:00:00

abstract：:Recently, significant differences between rats fed a casein diet and rats fed a soybean protein diet have been observed in hepatic phospholipid fatty acid patterns (Sjöblom, L., and Eklund, A., Biochim. Biophys. Acta 1004, 187-192, 1990). The influence of these two diets on the delta 6 desaturase activity was investig...

journal_title：Lipids

authors： Lindholm M,Eklund A

更新日期：1991-02-01 00:00:00

abstract：:Plaques and white matter from brains of multiple sclerosis (MS) patients were analyzed for lipid content, class composition, and fatty acid composition of total lipid, together with the fatty acid composition of plaque glycerophospholipids, and the results were compared with white matter from normal brain. Plaques con...

journal_title：Lipids

authors： Wilson R,Tocher DR

更新日期：1991-01-01 00:00:00

abstract：:A cross-fostering design was used to examine the effects on brain and behavioral development in mice of pre- and/or postnatal dietary supplementation with n-3 fatty acids. Pregnant mice were fed either of two liquid diets, control (con) or experimental (exp). Each diet provided 3% of the calories in the form of n-6 fa...

journal_title：Lipids

authors： Wainwright PE,Huang YS,Bulman-Fleming B,Mills DE,Redden P,McCutcheon D

更新日期：1991-01-01 00:00:00

abstract：:Rats were fed either a high alpha-linolenate diet or a high linoleate diet from weaning to 4 mon of age. Soybean oil was used as a control. Phospholipid compositions of erythrocytes from the three dietary groups were not significantly different. However, the difference in the alpha-linolenate (18:3n-3)/linoleate (18:2...

journal_title：Lipids

authors： Sakai K,Okuyama H,Kon K,Maeda N,Sekiya M,Shiga T,Reitz RC

更新日期：1990-12-01 00:00:00

abstract：:We describe a method for the quantitative analysis of molecular species of diacylglycerol and alkylacylglycerol as their diradylglycerobenzoate derivatives. Synthetic internal standards were used to provide quantitative determinations of the low levels of diacylglycerol and alkylacylglycerol and their individual molec...

journal_title：Lipids

authors： Warne TR,Robinson M

更新日期：1990-11-01 00:00:00

abstract：:The effect of phosphatidylserine and sterol carrier proteins on cholesterol exchange was determined using an assay not requiring separation of donor and acceptor membrane vesicles. Sterol carrier protein-2 (SCP2, also called nonspecific lipid transfer protein), but not fatty acid binding protein (FABP, also called ste...

journal_title：Lipids

authors： Schroeder F,Butko P,Hapala I,Scallen TJ

更新日期：1990-11-01 00:00:00

abstract：:The metabolic fate of high density lipoprotein (HDL) sphingomyelin in plasma was studied in rats over a 24-hr period after injection of HDL containing sphingomyelin which was 14C-labeled in the stearic (18:0) or lignoceric acid (24:0) moiety and 3H-labeled in the choline methyl groups. Decay of label in plasma followe...

journal_title：Lipids

authors： Bentejac M,Bugaut M,Delachambre MC,Lecerf J

更新日期：1990-10-01 00:00:00

abstract：:There is convincing evidence that normal cultured human keratinocytes possess a 15-lipoxygenase activity which, however, does not appear to manifest itself without cell membrane damage. When "activated", this enzyme transforms arachidonic acid into 15-hydroxyeicosatetraenoic acid (15-HETE), and linoleic acid into 13-h...

journal_title：Lipids

authors： Green FA

更新日期：1990-10-01 00:00:00

abstract：:The suppression of plasma very low density lipoprotein (VLDL) triglyceride levels by dietary fish oils rich in polyunsaturated n-3 fatty acids has been attributed to decreased hepatic VLDL secretion. To investigate the effect of n-3 fatty acids on lipid metabolism and VLDL secretion in a tissue culture system, we incu...

journal_title：Lipids

authors： Benner KG,Sasaki A,Gowen DR,Weaver A,Connor WE

更新日期：1990-09-01 00:00:00

abstract：:This report deals with the fluorometric determination of fatty alcohols generated by the reduction of the ester linkage of lipids with NaBH4, and with the limitations of the reduction method for assaying oxidized lipids. Optimum conditions for the fluorometric analysis of primary and secondary alcohols using 1-anthroy...

journal_title：Lipids

authors： Nakamura T,Maeda H,Takahashi Y,Hama Y

更新日期：1990-09-01 00:00:00

abstract：:The purpose of these experiments was to study the uptake and metabolism of exogenous triglyceride in the isolated perfused rabbit heart. When infused into the rabbit heart, [9,10-3H(N)]triolein was retained and incorporated into a lipid fraction that had the chromatographic mobility of authentic triolein. Incorporatio...

journal_title：Lipids

authors： Weis MT,Palazzo AJ,Williams JL Jr,Malik KU

更新日期：1990-08-01 00:00:00

abstract：:A quantitative method was developed for the assay of vitamin K in human colostrum and milk. The procedure combines preparative and analytical chromatography on silica gel in a nitrogen atmosphere followed by reversed phase high performance liquid chromatography (HPLC). Two HPLC steps were used: gradient separation wit...

journal_title：Lipids

authors： Canfield LM,Hopkinson JM,Lima AF,Martin GS,Sugimoto K,Burr J,Clark L,McGee DL

更新日期：1990-07-01 00:00:00

abstract：:The objective of the present studies was to examine the effect of dietary fat on the lipid composition of rat colonocytes and their utilization of short-chain fatty acids (SCFA). Rats were fed 14% beef fat, fish oil or safflower oil plus 2% corn oil in a semi-synthetic base diet for 4 wk. Colonocytes were isolated and...

journal_title：Lipids

authors： Awad AB,Ferger SL,Fink CS

更新日期：1990-06-01 00:00:00

abstract：:Rats were fed diets with three different ratios of alpha-linolenate (18:3 n-3) and linoleate (18:2 n-6), and then crescentic-type anti-glomerular basement membrane (GBM) nephritis was induced. The urinary protein levels and the plasma urea nitrogen levels were significantly higher, and histological abnormalities of gl...

journal_title：Lipids

authors： Watanabe S,Suzuki E,Kojima R,Suzuki Y,Okuyama H

更新日期：1990-05-01 00:00:00

abstract：:The catfish, Ictalurus punctatus, is an important model for studying the biochemical mechanisms of taste at the peripheral level. The type, amount and metabolic activity of the lipids within this tissue play important roles in taste transduction by forming the matrix in which the receptors for taste stimuli are imbedd...

journal_title：Lipids

authors： Rabinowitz JL,Huque T,Brand JG,Bayley DL

更新日期：1990-04-01 00:00:00

abstract：:We studied the actions of oxysterols on fatty acid distribution and lipid synthesis in cultured bovine aortic smooth muscle cells. Cultures were labeled with [1-14C]arachidonate or [1-14C]oleate. During a 24-hr incubation, 25- or 22R-hydroxycholesterol enhanced the incorporation of label into triglycerides, concomitan...

journal_title：Lipids

authors： Seillan C

更新日期：1990-03-01 00:00:00

abstract：:CDPcholine:1,2-diacylglycerol cholinephosphotransferase (EC 2.7.8.2) is located on the cytoplasmic side of the endoplasmic reticulum, and catalyzes the final step in the synthesis of phosphatidylcholine via the CDPcholine pathway. The enzyme was solubilized from hamster liver microsomes by 3% Triton QS-15, and partial...

journal_title：Lipids

authors： O KM,Choy PC

更新日期：1990-02-01 00:00:00

abstract：:In view of previous reports that the activity of the Mg(++)-dependent phosphatidic acid phosphatase in adipose tissues of rat and mouse is elevated in obesity, we attempted to assay this activity in biopsies of human omental adipose tissue obtained from normal-weight and morbidly obese subjects in connection with oper...

journal_title：Lipids

authors： Björkhem I,al-Shurbaji A,Backman L,Arner P

更新日期：1989-12-01 00:00:00

abstract：:The relationship between LCAT mediated HDL modification and the redistribution of lipoprotein-unassociated apoA-IV to HDL was investigated in vitro. Immunoaffinity-isolated rat lipoprotein-unassociated apoA-IV was added to apoB-, apoE-, apoA-IV depleted, [3H]cholesterol labelled rat plasma and incubated at 37 degrees ...

journal_title：Lipids

authors： Lefevre M,Goudey-Lefevre JC,Roheim PS

更新日期：1989-12-01 00:00:00

abstract：:Factors involved in reduced glutathione (GSH) and vitamin E-mediated inhibition of NADPH-dependent rat liver microsomal lipid peroxidation were examined. Lipid peroxidation was monitored over a time-course of 180 min by thiobarbituric acid reactive product formation. The addition of 5 mM GSH to the reaction system con...

journal_title：Lipids

authors： Graham KS,Reddy CC,Scholz RW

更新日期：1989-11-01 00:00:00

abstract：:We have studied the effect of various diets on the phospholipid fatty acid composition and in vitro delta 5 desaturase activity of hepatic microsomes derived either from the normal or streptozotocin-induced diabetic rat. The diets studied were the standard rat chow diet and a basal fat-free diet supplemented either wi...

journal_title：Lipids

authors： Dang AQ,Kemp K,Faas FH,Carter WJ

更新日期：1989-10-01 00:00:00

abstract：:Triacylglycerols containing linoleate and linolenate found in vegetable oils were synthesized in gram quantities for oxidation studies. Two acylation methods were examined to convert diacylglycerols or monoacylglycerols to the desired triacylglycerols. Acylations with fatty acid and 1,1'-dicyclohexylcarbodiimide in th...

journal_title：Lipids

authors： Awl RA,Frankel EN,Weisleder D

更新日期：1989-10-01 00:00:00